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KMID : 0616120120430010039
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Korean Journal of Pharmacognosy
2012 Volume.43 No. 1 p.39 ~ p.45
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Anti-inflammatory Effect of the Cirsium japonicum var. ussuriense 70% Ethanolic Extract in RAW264.7 Cells by Heme oxygenase-1 Expression
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Lee Dong-Sung
Kim Kyoung-Su
Li Bin
Choi Hyun-Gyu
Keo Samell
Jun Ki-young
Park Jun-Hyeong
Kim Youn-Chul
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Abstract
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Cirsium japonicum var. ussuriense has long been used in herbal medicine for the treatment of arthritis, dyspepsia, and bleeding in Korea. In the present study, we investigated anti-inflammatory effects of C. japonicum var. ussuriense against lipopolysaccharide(LPS)-induced activation in RAW264.7 cells by the expression of heme oxygenase (HO)-1. The 70% EtOH extract of the aerial parts of C. japonicum var. ussuriense (CJE), showed the potent anti-inflammatory effects on LPS-induced inflammation in RAW264.7 cells. The anti-inflammatory effect of CJE was demonstrated by the suppression of pro-inflammatory mediators, including pro-inflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2). Furthermore CJE induced HO-1 expression through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) and increased HO activity in RAW264.7 macrophages. The effects of CJE on LPS-induced NO and PGE₂ productions were partially reversed by an HO-1 inhibitor, tin protoporphyrin (SnPP). Therefore, it is suggested that CJE-induced HO-1 expression plays a role of the resulting anti-inflammatory effects in macrophages. These results suggest that CJE may be a promising candidate for the treatment of inflammatory diseases.
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KEYWORD
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Cirsium japonicum var. ussuriense, Heme oxygenase-1, RAW264.7, iNOS, COX-2, Inflammation
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